pe conjugated mouse anti chicken cd4  (SouthernBiotech)

Journal: Poultry Science

Article Title: Inhibitory effect of Eimeria maxima IFN-γ inhibitory molecules on the immune function of T cell subsets in chickens

doi: 10.1016/j.psj.2023.103098

Figure Lengend Snippet: Detection of the sorting purity of chicken CD8 + , CD4 + , CD4 + CD25 − and CD4 + CD25 + T cells by flow cytometry. (A) Magnetic bead sorting of chicken CD8 + T cells A1: blank cells (total PBMC); A2: CD8 + T cells before cell sorting; A3: CD8 + T cells after cell sorting. (B) Magnetic bead sorting of chicken CD4 + T cells B1: blank cells (total PBMC); B2: CD4 + T cells before cell sorting; B3: CD4 + T cells after cell sorting. (C) Magnetic bead sorting of chicken CD4 + CD25 − T cells C1: blank cells (total PBMC); C2: CD4 + CD25 − T cells before cell sorting; C3: CD4 + CD25 − T cells after cell sorting. (D) Magnetic bead sorting of chicken CD4 + CD25 + T cells D1: blank cells (total PBMC); D2: CD25 + cells before cell sorting D3: CD25 + cells after cell sorting D4: CD4 + CD25 + T cells before cell sorting; D5: CD4 + CD25 + T cells after cell sorting.

Article Snippet: The primary phycoerythrin ( PE )-conjugated mouse anti-chicken CD8α or CD4 antibody (Southernbiotech, Birmingham, AL) was added according to 1 × 10 8 cells.

Techniques: Flow Cytometry, FACS

Journal: Poultry Science

Article Title: Inhibitory effect of Eimeria maxima IFN-γ inhibitory molecules on the immune function of T cell subsets in chickens

doi: 10.1016/j.psj.2023.103098

Figure Lengend Snippet: Effects of rEmHPSP-2 and rEmHPSP-3 on the transcription of factors in chicken CD4 + CD25 − T cells and CD4 + T cells. (A) Effects of rEmHPSP-2 on the transcription of factors in chicken CD4 + CD25 − T cells. (B) Effects of rEmHPSP-3 on the transcription of factors in chicken CD4 + CD25 − T cells. (C) Differences in transcriptional level of cytokines of CD4 + T cells induced by rEmHPSP-2 and rEmHPSP-3 before and after CD25 + cells depletion. The differences between groups were analyzed using Duncan method of 1-way ANOVA, where the same letter indicates no significant difference ( P > 0.05) and different letter indicates significant difference ( P < 0.05). The differences between chicken CD4 + T cells with or without CD25 + cells in the protein group were analyzed using Independent sample t test. ns: P > 0.05; *: P ≤ 0.05; **: P ≤ 0.01; ***: P ≤ 0.001.

Article Snippet: The primary phycoerythrin ( PE )-conjugated mouse anti-chicken CD8α or CD4 antibody (Southernbiotech, Birmingham, AL) was added according to 1 × 10 8 cells.

Techniques:

Journal: Poultry Science

Article Title: Inhibitory effect of Eimeria maxima IFN-γ inhibitory molecules on the immune function of T cell subsets in chickens

doi: 10.1016/j.psj.2023.103098

Figure Lengend Snippet: Effects of rEmHPSP-2 and rEmHPSP-3 on the transcription of IL-10, TGF-β1 and CTLA-4 in chicken CD4 + CD25 + T cells. (A) Effects of rEmHPSP-2 on the transcription of IL-10, TGF-β1 and CTLA-4 in chicken CD4 + CD25 + T cells. (B) Effects of rEmHPSP-2 on the transcription of IL-10, TGF-β1 and CTLA-4 in chicken CD4 + CD25 + T cells. The differences between groups were analyzed using Duncan method of 1-way ANOVA, where the same letter indicates no significant difference ( P > 0.05) and different letter indicates significant difference ( P < 0.05).

Article Snippet: The primary phycoerythrin ( PE )-conjugated mouse anti-chicken CD8α or CD4 antibody (Southernbiotech, Birmingham, AL) was added according to 1 × 10 8 cells.

Techniques:

Journal: Vaccines

Article Title: Efficacy of Commercial Infectious Bronchitis Vaccines against Canadian Delmarva (DMV/1639) Infectious Bronchitis Virus Infection in Layers.

doi: 10.3390/vaccines10081194

Figure Lengend Snippet: Figure 4. Percentages of CD4+ (a) and CD8+ (b) T cells in peripheral blood mononuclear cells (PBMCs) at 5 and 12 dpi following infection with the Canadian DMV/1639 strain (IBV/Ck/Can/17– 036989). Mean percentages of CD4+ and CD8+ T cells were compared between groups using one-way ANOVA test followed by Tukey’s multiple comparisons test, and the error bars represent the SD.

Article Snippet: After centrifugating the cells as indicated above, the supernatant was discarded and the cell pellets were resuspended in the dark for 20 min at 4 ◦C using fluorescein isothiocyanate (FITC)-conjugated mouse anti-chicken CD8 (Southern Biotech, Birmingham, AL, USA) and phycoerythrin (PE)-conjugated mouse anti-chicken CD4 (Southern Biotech, Birmingham, AL, USA).

Techniques: Infection